Posted at 02 December 2020
Numerous different in vitro assays, labeled as “antioxidant assays,” are used intensively to predict the antioxidant capacity of phenolic compounds. Most of these methods give valuable information in terms of chemical reactivities but also present some weaknesses. It may be difficult to use them to predict antioxidant capacity in real conditions. Indeed, lipid oxidation is a complex reaction, with numerous paths and components, and antioxidant action can occur via a multitude of mechanisms, especially when different phases coexist in the lipid‐based formulation.
Yet, correctly combining selected in vitro methods to extract complementary information with respect to antioxidant behaviors would be much better and will help reducing the gap between prediction and efficacy in the finished product.
Thus, we hereby propose a methodology to evaluate the antioxidant properties of 12 selected natural polyphenolic extracts based on the appropriate combination of in vitro assays. The scores obtained with the DPPH test, the measure of the chelation capacity, the evaluation of antioxidants efficiency in emulsions (CAT and VESICAT assays), or in bulk oils were submitted to a statistical treatment.
This analysis allowed a ranking on their global antioxidant capacities and the creation of clusters depending on their mechanisms of action and the type of media where the tests were performed.
'Journal of the American Oil Chemists' society - October 2020
Authors: Charlotte Deyrieux1, Erwann Durand1, Sonia Guillou2, Nathalie Barouh1, Bruno Baréa1, Françoise Michel Salaun2, Pierre Villeneuve1
1 - CIRAD, UMR IATE, Montpellier, France
2 - Diana Pet Food, Elven, France
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