Опубликовано на 19 сентября 2018

One of the first indicator of oxidation in oils and fats is the presence of hydroperoxides, therefore, it is important to find a reliable method to assess these oxidation products. Iodometric titration is commonly used to determine lipid hydroperoxide (LOOH) concentrations. Although this method is simple to set up, it is labor-intensive, time consuming, and uses a lot of solvents. 
TPP/TPPO assay coupled with FTIR-ATR spectroscopy is a good alternative to the iodometric method since it allows a fast and robust hydroperoxides measurement, with minimal use of organic solvents. This rapid peroxide value determination can be adapted for routine quality control analysis, especially for oxidation in real-time and accelerated aging assays to evaluate oxidative stability during shelf-life, for industrial purpose or lab scale experiments.